Skeletal muscle mitochondrial DNA content in exercising humans

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Skeletal muscle mitochondrial DNA content in exercising humans.

Several weeks of intense endurance training enhances mitochondrial biogenesis in humans. Whether a single bout of exercise alters skeletal muscle mitochondrial DNA (mtDNA) content remains unexplored. Double-stranded mtDNA, estimated by slot-blot hybridization and real time PCR and expressed as mtDNA-to-nuclear DNA ratio (mtDNA/nDNA) was obtained from the vastus lateralis muscle of healthy human...

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Decline in skeletal muscle mitochondrial function with aging in humans.

Cumulative mtDNA damage occurs in aging animals, and mtDNA mutations are reported to accelerate aging in mice. We determined whether aging results in increased DNA oxidative damage and reduced mtDNA abundance and mitochondrial function in skeletal muscle of human subjects. Studies performed in 146 healthy men and women aged 18-89 yr demonstrated that mtDNA and mRNA abundance and mitochondrial A...

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BACKGROUND Exhausting exercise reduces the mitochondrial DNA (mtDNA) content in the skeletal muscle of healthy subjects due to oxidative damage. Since patients with chronic obstructive pulmonary disease (COPD) suffer enhanced oxidative stress during exercise, it was hypothesised that the mtDNA content will be further reduced. OBJECTIVE To investigate the effects of exercise above and below th...

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Skeletal muscle mitochondrial function and lean body mass in healthy exercising elderly.

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ژورنال

عنوان ژورنال: Journal of Applied Physiology

سال: 2005

ISSN: 8750-7587,1522-1601

DOI: 10.1152/japplphysiol.00289.2005